Usefulness of microsatellite typing in population genetic studies of Trypanosoma cruzi
نویسندگان
چکیده
منابع مشابه
Usefulness of microsatellite typing in population genetic studies of Trypanosoma cruzi.
Through microsatellite analysis of 53 monoclonal populations of Trypanosoma cruzi, we found a remarkable degree of genetic polymorphism with no single multilocus genotype being observed more than once. The microsatellite profile proved to be stable during 70 generations of the CL Brener clone in culture. The microsatellite profiling presented also high diagnostic sensitivity since DNA amplifica...
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متن کاملMultilocus Sequence Typing (MLST) for Lineage Assignment and High Resolution Diversity Studies in Trypanosoma cruzi
BACKGROUND Multilocus sequence typing (MLST) is a powerful and highly discriminatory method for analysing pathogen population structure and epidemiology. Trypanosoma cruzi, the protozoan agent of American trypanosomiasis (Chagas disease), has remarkable genetic and ecological diversity. A standardised MLST protocol that is suitable for assignment of T. cruzi isolates to genetic lineage and for ...
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ژورنال
عنوان ژورنال: Memórias do Instituto Oswaldo Cruz
سال: 2001
ISSN: 0074-0276
DOI: 10.1590/s0074-02762001000300023